Esterase electrophoresis of Clostridium perfringens bovine strains

L. BALDASSI(1), M. L. BARBOSA(2), M. M. BACH(3), S. T. IARIA(4)

(1)Laboratório de Bacteriologia Geral e Micobacterioses, Instituto Biológico, (2)Instituto Adolfo Lutz, (3)Instituto Biológico - UNINOVE, (4)Departamento de Microbiologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, USP.

ABSTRACT: Eighty-nine of 144 isolates of Clostridium perfringens obtained from 187 samples of 71 bovine in several Brazilian states were submitted to esterase electrophoresis for typing. Mobilites electrophoresis, as parameter, were settled down by isolates from ATCC pattern of types A, B, C, and D. Of the 89 isolates, 43 (48.3%) were characterized as electrophoretic type A, 20 (22.5%) as D, 18 (20.2%) as C, and 3 (3.4%) as B. Five (5.6%) isolates did not identity with any type.  Similarly, esterase electrophoresis enabled the typing of 94.4 % of the isolates, demonstrating to be an appropriate method for animal sample analyses.

KEY WORDS: Clostridium perfringens, bovine, electrophoresis, esterase

CORRESPONDENCE TO:

L. Baldassi, Laboratório de Bacteriologia Geral e Micobacterioses, Instituto Biológico, Avenida Conselheiro Rodrigues Alves, 1252, CEP 04014-002, São Paulo, SP, Brasil, Email: baldassi@biologico.sp.gov.br