PRELIMINARY RESULTS FROM RENAL ALTERATIONS PROMOTED BY Bothrops erythromelas VENOM ANALYZED IN THE ISOLATED PERFUSED RAT KIDNEY METHOD

Sousa, F.C.M., Havt, A., Barbosa, P.S.F., Martins, A.M.C., Angelim, E.V., Nobre, A.C.L., Facó, P.E.G., Guarnieri, M.C., Fonteles, M.C., Monteiro, H.S.A.

Federal University of Ceará, Brazil. 

Renal alterations promoted by ophidian accidents promote severe damages that can be cause death. Our goal is identify the possible nephrotoxic effects promoted by Bothrops eryhtromelas venom in the isolated perfuse drat kidney method. Wistar rats were anesthetized with sodium pentobarbitone (50mg/mL), and their kidneys were surgically removed following Fonteles and coworkers (Am. J. Physiol. 244, 235-346, 1983) technique. We used a Krebs-Henseleit perfusion solution modified by serum bovine albumin (6g%). Each experiment (n=2) lasted 120 minutes. The first 30 minutes of each perfusion were used as internal control (IC) and compared (Student t Test, *p<0,05) to other 3 treated (10 mg/mL) periods (T) of 30 minutes, that were named 60, 90 and 120 minutes. The most intense effects were seen in the 120 minutes period. Bothrops erythromelas venom caused intense decreases in the perfusion pressure (IC₃₀ = 109.9 ± 0.44, T₉₀ = 72.03 ± 3.65, T₁₂₀ = 84.53 ± 0.60 mmHg) and renal vascular resistance (IC₃₀ = 4.54 ± 0.017, T₉₀ = 2.97 ± 0.15, T₁₂₀ = 3.50 ± 0.024 mmHg/g/min/mL). Urinary flow was reduced at the 60 minutes period but at 120 minutes we found an intense increase. Glomerular filtration rate was also reduced at the 60 minutes period. Sodium tubular transport (IC₃₀ = 80.90 ± 0.56, T₉₀ = 58.96 ± 2.10, T₁₂₀ = 58.68 ± 2.32 %) and potassium tubular transport (IC₃₀ = 62.99 ± 1.64, T₉₀ = 52.67 ± 1.44, T₁₂₀ = 53.36 ± 1.44 * mmHg) were reduced in the periods named 90 and 120 minutes. The Bothrops erythromelas venom caused an intense decrease in the vascular parameters which was independent of the increase of urinary flow. Maybe the reductions for sodium and potassium tubular transport had intensified the alterations for urinary flow in the end of each perfusion.

Supported by: CAPES

CORRESPONDENCE TO:

Alexandre Havt, Rua Tenente Benévolo 1560, Apto. 802, Fortaleza, CE, CEP: 60160-210, Brasil, Email: havt@speeder.com.br