STUDY OF COMPARATIVE ANESTHESIC REACTIONS WITH KETAMINE AND KETAMINE-MIDAZOLAN IN SERPENTS OF THE SPECIES Crotalus durissus terrificus

SILVA, A.M.(1,), RIBEIRO, W.(1), LOPES MARTINS, R.A.B.(1), PRIANTI A.C.G.(1), CRUZ, M.L.(2),  COGO, J.C.(1).

(1)Universidade do Vale do Paraíba – UNIVAP, Avenida Shishima Hifumi, 2911, Urbanova, CEP 12244-000, S. J. Campos, SP, (2)Universidade Estadual Paulista, UNESP, Botucatu, SP.

Experimental scientific studies involving serpents demand research of alternatives to offer more safety in the manipulation. Equally, the present work is aimed at obtaining safe manipulation for serpents of the Crotalus durissus terrificus species, using anesthesic substances ketamine (KTM) and its association ketamine/midazolan (KTM/MDZ). Ten adult specimens of Crotalus durissus terrificus were used (492 ± 123 gr.), collected in the river Paraíba valley area, maintained in the Serpentarium of the University of the Valley of Paraíba - UNIVAP, kept at a constant temperature of 26ºC and divided in 2 groups of 5 animals each. Group I – intramuscular injection KTM (100mg/ml), in doses of 80 mg/kg. Group II – Intramuscular injection KTM (100mg/ml), in doses of 80mg/kg,  in the same syringe with MDZ (5mg/ml), in the dose of 0,2 weight mg/kg. To evaluate the pharmacological answer during the anesthesic process, different physiologic parameters (breathing frequencies, heart rate, pain sensitivity, time of apnea and rectal temperature) and behavior (displacement, exhibition of the tongue, coilability, rattle, bite, and escape) were observed. In relation to the physiologic parameters we can conclude that there were not significant differences between KTM and KTM/MDZ, except when evaluated the intensity of pain sensitivity parameter after mechanical stimulation. As, we can conclude that the association KTM/MDZ showed a mean of 1,4 (n=5) on a scale of 0 - 4,0 time vs intensity (240 min) (p<0,001), although when compared with KTM showed a mean of 3,0 (n=5). It is believed that this is related to the rapid effect of this chemical class. Based on the data moderated in the literature, we didn't observe the same pharmacological profile for KTM. This way the job of KTM/MDZ presents an action profile that makes it possible to have safe manipulation, resulting from the muscle relaxation effect promoted by Benzodiazepinic and analgesic/anesthesic of KTM.

CORRESPONDENCE TO:

SILVA, A.M., Universidade do Vale do Paraíba - UNIVAP. Avenida Shishima Hifumi, 2911, Urbanova, CEP 12244-000, São José dos Campos, S.P., Brasil, Email: mas@univap.br