Poster 25 - Action of anti-bothropic factor isolated of Didelphis marsupialis on renal effect

Poster 25. Congresso da Sociedade Brasileira de Toxinologia, 8., Symposium of the Pan American Section of the International Society on Toxinology, 8., 2004, Angra dos Reis, Brasil. Abstracts... J. Venom. Anim. Toxins incl.Trop. Dis., 2004, 10, 3, p.384.

Action of Anti-bothropic Factor Isolated of Didelphis marsupialis on Renal Effect of Bothrops erythromelas Venom.

¹Sousa, F.C.M., ²Martins, A.M.C., ³Toyama, M. H., ¹Havt, A., ²Aprígio, C.C., ¹Barbosa, P.S.F., ¹Monteiro, J.S.A.M., ²Queiroz, M.G.R., ¹Fonteles, M.C., ¹Monteiro, H.S.A.

1 Depto. de Fisiologia e Farmacologia e 2 Depto. de Análises Cínicas e Toxicológicas - UFC. 3 Depto. de Bioquímica-UNESP.

The Bothropserythromelas is responsible for much snakebite in Northeastern Brazil and induce acute renal failure. The Didelphis marsupial serum inhibed myonecrotic, hemorrhagic, lethal, edematogenic and hyperalgesic effects observed in envenomation by ophidian venom. In the present study, we examined the action of anti-bothropic factor isolated of Didelphis marsupialis on renal effects of B. erythromelas (VBE). Isolated kidneys from Wistar rats, weighing 240 to 280g, were perfused with Krebs-Henseleit solution containing 6% of bovine serum albumin. The parameters studied included perfusion pressure (PP), renal vascular resistance (RVR), glomerular filtration rate (GFR), urinary flow (UF), percent of sodium tubular transport (%TNa⁺) and percent of potassium tubular transport (%TK⁺). The B. erythromelas venom (10 µg/mL) decreased the PP (ct = 108,69 ± 5,1 mmHg vs VBE = 65,2 ± 5,6 mmHg*) and RVR (ct = 5,76 ± 0,65 mmHg/ ml.g^-1. min^-1 vs VBE = 3,10 ± 0,45 mmHg/ ml.g^-1. min^-1*). On the other hand, the GFR decreased at 60 min. (ct = 0,761 ± 0,07 ml .g^-1. min^-1 vs VBE = 0,422 ± 0,12 ml.g^-1.min^-1*) and increased at 120 min (ct = 0,718 ± 0,08 ml.g^-1. min^-1 vs VBE = 1,236 ± 0,25 ml.g^-1.min^-1*). The UF (ct = 0,138 ± 0,01 ml.g^-1. min^-1 vs VBE= 0,361 ± 0,08 ml.g^-1. min^-1*) increased significantly when compared with control group. The venom reduced the %TNa⁺ (ct = 79,76 ± 0,56 vs VBE = 59,24 ± 3,15*) and %TK⁺ (ct = 63,83 ± 5,59 vs VBE = 58,07± 3,8*). The anti-bothropic factor from Didelphis marsupialis (30µg/mL) incubed with B. erythromelas venom (10µg/mL) reversed the effects on RVR, UF, GFR, % TNa⁺ and %TK⁺. In conclusion, B. erythromelas venom altered all the renal functional parameters evaluated and the anti-bothropic factor from D.marsupialis was able of inhibed the effects induced by venom in isolated kidney.