Poster 26.  Congresso da Sociedade Brasileira de Toxinologia, 8., Symposium of the Pan American Section of the International Society on Toxinology, 8., 2004, Angra dos Reis, Brasil.  Abstracts...  J. Venom. Anim. Toxins incl.Trop. Dis., 2004, 10, 3, p.385.

 

Effects Of Bothrops marajoensis Venom In The Isolated Perfused Rat Kidney

 

1Sousa,T.M.; 1Martins,A.M.C.; 1Evangelista,J.S.A.M.; 1Barbosa,P.S.F.; 1Martins,R.D.; 1Amora,D.; 1Alves,R.S.; 1Porto,M.A.; 2Toyama, M.H.; 3Fonteles,M.C.; 1Monteiro,H.S.A.

 

1 Depto Fisiologia e Farmacologia, Universidade Federal do Ceará - Brasil; 3 Universidade Presbiteriana Mackenzie,SP - Brasil; 2 UNESP, SP – Brasil.

 

Bothropsmarajoensis is a snake found in the “Marajó” Brazilian isle. The aim of this study was to identify the renal alterations caused by its venom, since one of the most common complications in cases of ophidian bites is acute renal failure.The effects of Bothropsmarajoensis (Bm) venom (10mg/ml) were examined using the isolated perfused  kidney from Wistar rats, as it was described by Fonteles, M.C. et al, Am. J. Physiol., 244, p.235, 1983. All experiments were preceded by a 30 minutes internal control period and an external control group (C), in which the kidneys were perfused only with Krebs-Henseleit solution containing 6g% of a previously dialysed bovine albumine serum. The data was analyzed by Student t-test and the level of significance was set at *p<0,05. The venom showed significant decrease in the perfusion pressure (C:108,69±4,6; Bm:69,8±4,2 mmHg*) and renal vascular resistance (C:5,41±0,33; Bm:3,27±0,2 mmHg/mL.g-1.min-1*) in 90 and 120 minutes of the beginning of the experiment. It also caused decrease in the urinary flow (C:0,16 ±0,1; Bm: 0,083 ±0,021 mL/min/g*) in 90min, followed by an increase in 120min (C:0,17±0,1; Bm:0,24 ±0,03 mL/min/g*), although the glomerular filtration rate kept significant decreased until the end of the experiment (C:0,78±0,07; Bm:0,605 ±0,08 mL/min/g*). It decreased the percent of proximal and total tubular transport of sodium (C:80,17±2,62; Bm:55,39±3,23%*) and chloride (C:77,33±0,41; Bm:55,36±3,3%*). The venom from Bothrops marajoensis caused significant renal alterations. Some of its effects are similar to others botropic venoms, like Bothropsjararacussu and B. moojeni. Further studies are needed to show if these are direct or indirect renal effects.

 

Financial support: CNPq; CAPES; FUNCAP.

 

Correspondence to: serrazul@baydenet.com.br