Poster 38.  Congresso da Sociedade Brasileira de Toxinologia, 8., Symposium of the Pan American Section of the International Society on Toxinology, 8., 2004, Angra dos Reis, Brasil.  Abstracts...  J. Venom. Anim. Toxins incl.Trop. Dis., 2004, 10, 3, p.397.

 

 

Action of Anti-bothropicFactor Isolated of Didelphis marsupialison Renal Effect caused by myotoxinI ( Lis49) of Bothrops jararacussuVenom.

 

1Barbosa, P.S.F., 1Abreu, J.P.S., 2Martins, A.M.C., 3Toyama, M. H., 1Havt, A., 1Nobre, A.C.L., 3Maragoni, S., 2Martins, R.D., 2Alves, R.S., 2Queiroz, M.G.R., 1Fonteles, M.C., 1Monteiro, H.S.A.

 

1 Depto. de Fisiologia e Farmacologia e 2 Depto. de Análises Cínicas e Toxicológicas - UFC. 3 Depto. de Bioquímica-UNESP.

 

The myotoxin ( BthTxI) of Bothropsjararacussu venom is protein Lys 49 PLA2 devoid of enzymatic activity. The Didelphismarsupial serum inhibed myonecrotic, hemorrhagic, lethal, edematogenic and hyperalgesic effects observed in envenomation by ophidian venom. In the present study, we examined the action of anti-bothropic factor isolated of Didelphismarsupialis on renal effects caused by the myotoxin I (BthTxI). Isolated kidneys from Wistar rats, weighing 240 to 280g, were perfused with Krebs-Henseleit solution containing 6% of bovine serum albumin. The parameters studied included perfusion pressure (PP), renal vascular resistance (RVR), glomerular filtration rate (GFR), urinary flow (UF), percent of sodium tubular transport (%TNa+) and percent of potassium tubular transport (%TK+). The myotoxin I of B. jararacussu venom (5 µg/mL) increased the PP (ct = 110,1 ± 3,7 mmHg vs MthTxI = 171,2 ± 6,3 mmHg*), RVR (ct = 5,49 ± 0,54 mmHg/ ml.g-1. min-1 vs MthTxI = 8,62 ± 0,37 mmHg/ ml.g-1. min-1 *), GFR  (ct = 0,718 ± 0,10 ml .g-1. min-1 vs MthTxI = 0,855 ± 0,13 ml.g-1.min-1*)  and UF (ct = 0,140 ± 0,006 ml.g-1. min-1 vs BthTXI= 0,326 ± 0,05 ml.g-1. min-1*)  The myotoxin I of  B. jaracussu venom reduced the %TNa+ (ct = 79,76 ± 0,56 vs MthTxI = 62,23 ± 4,12*) and  %TK+ (ct = 66,80 ± 3,69 vs MthTxI = 55,79± 5,57*). The anti-bothropic factor from Didelphismarsupialis (30µg/mL) incubed with myotoxin I of B. jararacussu venom (5µg/mL) reversed the effects on  PP, RVR, UF, GFR, % TNa+ and %TK+. In conclusion, the anti-bothropic factor from D.marsupialiswas able of inhibed the effects induced by myotoxin I of Bothropsjararacussu venom in isolated kidney.

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