Pharmacology Blocked of the Renal Effect Caused by Lectin from Bothrops moojeni

^{1Barbosa, P.S.F., 1Abreu, J.P.S.}, ²Martins, A.M.C., ³Toyama, M. H., ¹Havt, A., ¹Nobre, A.C.L., ³Toyama. D.O., ³Maragoni, S., ²Martins, R.D., ²Alves, R.S., ²Queiroz, M.G.R., ¹Fonteles, M.C., ¹Monteiro, H.S.A.

1 Depto. de Fisiologia e Farmacologia e 2 Depto. de Análises Cínicas e Toxicológicas - UFC. 2 Depto. de Bioquímica-UNESP.

Lectin from B. moojeni (BmL) is protein with one carbohydrate-binding site (galactose). The aim of present work was blockage of the renal effect caused by lectin from Bothropsmoojeni. Isolated kidneys from Wistar rats (n=6), weighing 240 to 280g, were perfused with Krebs-Henseleit solution containing 6% of bovine serum albumin. The parameters studied included perfusion pressure (PP), renal vascular resistance (RVR), glomerular filtration rate (GFR), urinary flow (UF), percent of sodium tubular transport (%TNa⁺) and percent of potassium tubular transport (%TK⁺). The lectin from B. moojeni venom (10 µg/mL) increased the PP (ct = 110,1 ± 3,7 mmHg vs BmL = 152,5 ± 2,1 mmHg*), RVR (ct = 5,49 ± 0,54 mmHg/ ml .g^-1. min^-1 vs BmL = 7,77 ± 0,30 mmHg/ ml.g^-1. min^-1 *), GFR  (ct = 0,718 ± 0,10 ml .g^-1. min^-1 vs BmL = 1,184 ± 0,09 ml.g^-1.min^-1*)  and UF (ct = 0,140 ± 0,006 ml.g^-1. min^-1 vs BmL= 0,237 ± 0,013 ml.g^-1. min^-1*). The indomethacin (10 µg/mL) (inhibitor non selective of ciclooxygenase) reverted the effect caused by lectin from B. moojeni in PP, RVR, RFG e UF. In conclusion, lectin from B. moojeni venom altered all the renal functional parameters evaluated and the indomethacinwas able of inhibed the effects induced by venom in isolated kidney.

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