Poster 065 - Antibacterial and anti-PLA2 activities of Musa Paradisiaca extract and its isolated

Poster 65. Congresso da Sociedade Brasileira de Toxinologia, 8., Symposium of the Pan American Section of the International Society on Toxinology, 8., 2004, Angra dos Reis, Brasil. Abstracts... J. Venom. Anim. Toxins incl.Trop. Dis., 2004, 10, 3, p.424.

Antibacterial AndAnti-PLA2 Activities Of Musa Paradisiaca Extract And Its Isolated Fractions.

1Borges, M. H.; ²Alves, D. L. F.; ^4Diniz, C. G.; ⁴Carvalho, M. A. R., ⁴Farias, L. M., Da Silva, M. C. ³Piló-Veloso, D., ¹De Lima, M. E.

1 Lab. Ven. e Tox. Animais, Dep. Bioq. e Imun., 2 Dep. Farmac., ICB, 3 Dep. Quím., ICEx, 4 Lab. de Microb. Oral e Anaer. do ICB/UFMG, Av. Antônio Carlos, 6627, 31270-901, Belo Horizonte-MG, Brazil.

Banana plant has many biologically active compounds that are used in folkloric medicine, to treat several pathological conditions, such as snakebite. Phospholipases A2 (PLA2) are found in snake venoms and in biological fluids. They catalyze the hydrolysis of sn-2 ester bonds of phospholipids, releasing free fatty acids and lysophospholipids that can act as second messenger or as inflammatory mediators. Plant extracts have been show the antibacterial and antifungical activities too. Tannins found in several plants are very effective to treat infections caused by microorganisms. The antibacterial mechanism may be related to its astringent properties and its interaction with membranes of the microorganisms. In this work we examined the action anti-PLA2 and antibacterial of Musa paradisiaca (“banana prata”) stem juice and its fractions. Bothropsjararacussu and Crotalusdurissus terrificus venoms and Crotoxin, from C. d. terrificus venom were utilized as reference assays of PLA2 activity The aqueous extract of M.paradisiaca and its fractions were mixed with venoms/toxin for measure their influence on PLA2 activity. The ratio of protein/Musaparadisiaca was 1:1 (w:w). For antibacterial test the strains used were Staphylococcus aureus (ATCC 29213.), Staphylococcus aureus(ATCC 33591), Pseudomonas aeroginosa (ATCC 10145), Staphylococcus epidermidis (ATCC 12228), Escherichia coli (ATCC 25922) and Enterococcusfaecalis(ATCC 19433). The microorganisms were cultured on BHI (Brain Heart Infusion) supplemented with yeast extract (5%). The agar diffusion method was performed for screening the antibacterial activity. M. paradisiaca extract and F1-F14 HPLC (500 mg/mL) fractions were applied on the paper discs (0.5 mm diameter) and added to petri plates previously incubated with each bacterial strain. After 24 h at 37°C, the inhibition zone was evaluated. M. paradisiaca, F5 and F6 inhibited 100% of PLA2 activity of these venoms. F6 fraction inhibited the growth of S. aureus, P. aeroginosa and S. epidermidis. F3 and F4 inhibited S. epidermidis, too. Based on the fact that both PLA2 enzymes and microorganisms can be present in pathological conditions, the utilization of tannins for inhibition of microbial growth and PLA2 enzymes may reveal useful clinical applications.