Poster 66.  Congresso da Sociedade Brasileira de Toxinologia, 8., Symposium of the Pan American Section of the International Society on Toxinology, 8., 2004, Angra dos Reis, Brasil.  Abstracts...  J. Venom. Anim. Toxins incl.Trop. Dis., 2004, 10, 3, p.425.

 

 

A Study On Biological Activites Of Bothrops cotiara (“Cotiara”, “Mboi-cotiá”, “Cotiarinha”, “Black Bellied Yarará”) And Its Neutralization By Therapeutic Antivenoms.

 

1de Roodt, A.R.; 2Estévez, J.; 1Dolab, J.A.; 1Manzanelli, V.M.; 1Piñeiro, N.; 3Paniagua-Solis, J.F.; 4Vogt, A.U.

 

1 Instituto Nacional de Producción de Biológicos, Administración Nacional de Laboratorios e  Institutos de Salud “Dr. Carlos G. Malbrán”, Ministerio de Salud. 2 Instituto Bioclón, México DF, México. 3 Laboratorios Silanes, México DF, México. 4 Centro Zootoxicológico de Misiones, Oberá, Misiones, Argentina.

 

Bothropcotiara is a venomous snake of sporadic finding in the province of Misiones in Argentina, South of Brazil and Paraguay. Data on the clinics of the envenomation produced by the bite of this snake and those on the study of its venom are scarce. In addition there are no information on the neutralizing capacity of the antivenoms for therapeutic use available in this region on the toxicity of this venom.  In spite of these facts, the lethal potency, hemorrhagic, necrotizing, coagulant and prothrombinic, defibrinogenating, indirect hemolytic and fibrinolytic activities of the venom of Bothropscotiara specimens from the province of Misiones were studied. In addition the electrophoretic and chromatographic profiles and some toxic activities of the venom fractions were studied. The toxic activities were in the range of those described for the other Bothrops species from Argentina and the electrophoretic and chromatographic studies showed similarities with those described for the other bothropic venoms. The immunochemical reactivity of six South American anti Viper antivenoms was studied by ELISA showing a very strong reactivity with all the antivenoms studied. The neutralizing capacity of three of these therapeutic antivenoms was studied on lethal potency and hemorrhagic, necrotizing, coagulant, prothrombinic and hemolytic activities showing a very close neutralizing capacity. Data obtained in this study strongly suggest that the antivenoms for therapeutic use available in this area of South America are useful to neutralize the toxic and enzymatic activities of the venom of  this uncommon specie of Bothrops.

 

Correspondence to: aderoodt@uolsinectis.com