Bothrops neuwiedi venom induces increases vascular permeability.

AYMORE, S., Moreira, K. G.,Magalhães, M. R., Peres, M. C., Da Silva, N.J.

Centro de Estudos e Pesquisa Biológicas, Universidade Católica de Goiás

Edema formation is a common feature of cutaneous inflammatory response and is dependent on a synergism between mediators that increase vascular permeability and those that increase blood flow (Williams and Peak, 1977; Williams, 1979; Brian and Williams, 1985). The ability of venoms from snakes of  the Bothrops genus to induce edema and hemorrhage is well known due to the influence of endogenous mediators, among others, contained in the venom (Trebien and Calixto, 1989; Silva et al.,1995).The increasing of the vascular permeability and edematogenic activity was induced in male Swiss mice (n=6/8) by subplantar injection of Bothrops neuwiedi  venom (0,56; 1,125; 2,25; 4,5 and 9mg in 20ml saline / paw). Evans blue dye was injected (0,1ml/mice) and paw weight increase and dye extravasation was used for quantification of edema and the vascular permeability. The edema reached a maximum within 2 hours after the venom injection and progressively decreased after this period, diminishing completely by 24 hours. The minimum edematogenic dose was 0,56mg and all the used doses were statistically significant (16,56 ± 2,72; 26,74 ± 2,56; 27,68 ± 2,76; 27,98 ± 4,23; 29,60 ± 3,73, % of extravascular dye ± SEM). The results indicated that the venom from Bothrops newiedi induced a significant increase in the extravascular permeability and edema.

Correspondence to: sayonaraa@ig.com.br