Antibodies Production and Histopathological Analyses in Rabbits Experimentally Envenomation by Philodryas olfersii and Philodryas patagoniensis venoms

Rocha, M.M.T.¹, Paixão-Cavalcante, D.², Tambourgi, D.V.² and Furtado M.F.D.¹

1 Laboratório Herpetologia - I.B. 2 Laboratório Imunoquímica - I.B.

In Brazil, about 40% of ofidic accidents are caused by considered non-poison snakes. The snakes of Philodryas  genera  are the most important among then, by their clinical aspects. This accidents are characterized by oedema, haemorrhage and local painful, that some times can be misidentified like botropic envenomation. Searching for a specifc treatmant for this accidents, rabbits were inoculated with Philodryasolfersii (P.o.) or Philodryaspatagoniensis (P.p.) venoms and the histological paterns antibodies production were evaluated. Skin histopathologic analyses from P.olfersii  or P. patagoniensis  of inoculated rabbits show normal epidermis, intense hemorrhage in dermis and muscle fibers lesion. Serum against P.o. or P.p.  produced in rabbits had cross-reactive between then when analyzed by ELISA and immunoblotting. These venoms provoked a skin lesion, experimentally, in inoculated rabbits and they were able to induce antibodies production with capacity to recognize the venom components of both species of Philodryas.

Correspondence to: danipaixao@butantan.gov.br