Poster 217 - Citotoxic, and melanogenic activity of Brosimum gaudichaudii extract over B16F10

Poster 217. Congresso da Sociedade Brasileira de Toxinologia, 8., Symposium of the Pan American Section of the International Society on Toxinology, 8., 2004, Angra dos Reis, Brasil. Abstracts... J. Venom. Anim. Toxins incl.Trop. Dis., 2004, 10, 3, p.576.

Citotoxic,and Melanogenic Activity of Brosimum gaudichaudii Extract Over B16F10 Murine Melanon Culture.

¹Rosa, F. C.; ²Nomizo, A.; ¹Couto, L. B; ¹Lourenço, M. V.; ¹Malta Neto N. R.; ²De Marco, N.; ¹França, S. C.

1 Depto. Biotecnologia, Universidade de Ribeirão Preto - UNAERP; 2 Depto. de Análises Clínicas, Toxicológicas e Bromatológicas, Faculdade de Ciências Farmacêuticas - FCFRP-USP, Ribeirão Preto, Brasil.

Brosimumgaudichaudii is a plant native to the Brazilian cerrado. Among a variety of substances isolated from this species are the furanocumarins psoralen and bergapten. Furanocumarins have been used for the treatment of skin diseases as vitiligo. The present study was carried out to evaluate the melanogenic and “in vitro” citotoxicity of crude water extracts of Brosimum gaudichaudii root bark, over murine melanom cultured cells (ATCC B16F10). Cells were cultured with different concentrations of the extracts for 48-72h periods. After incubation, cells were lysed with 1M NaOH and the melanin contents were measured by spectrometric analysis at 450nm. Citotoxic activity was investigated according to the Mosmann colorimetric method (MTT) using tetrazolium salt. Obtained results showed that both, lipo soluble fractions of B. gaudichaudii extracts and one of its isolated compound the bergapten within the range 0.5-0.1 mg/mL and 50-100 mg/mL, respectively, induced melanogenesis. In addition, lipo soluble fractions as well as hydro soluble fractions of B. gaudichaudii extracts, at concentration over 0.1mg/mL were citotoxic for tumoral B16F10 cells while the hydro soluble fraction at concentration lower than 0.01mg/mL induced proliferation of melanom B16F10. Performed assays also showed that 25-200 mg/mL of bergapten induced dose dependent proliferation of melanom B16F10. Overall data confirm the efficacy of B. gaudichaudii root bark extract as a phytomedicine to treat pigmentation skin diseases, due to its property to enhance melanin production. Additionally, the extracts exhibited cytotoxic activity against melanom cells which are resistant to most of the chemotherapeutic agents utilized.