Received: November 8, 2004, Accepted: February 4, 2005, Published online: July 1, 2005.

VIPOXIN SPECIFICITY STUDIED BY GAS CHROMATOGRAPHIC DETERMINATION OF ENZYMATIC REACTION PRODUCTS.

INFLUENCE OF Ca2+, Mg2+ AND Sr2+

ATANASOV V. (1), BARDAROV V. (2), ALEKSIEV B. (3), MITEWA M. (1)

(1) Department of Analytical Chemistry, Faculty of Chemistry, University of Sofia, Sofia, Bulgaria; (2) Toxicochemical Laboratory, Department of Toxicology, Military Medical Academy, Sofia, Bulgaria; (3) Department of Organic Chemistry, University of Chemical Technology and Metallurgy, Sofia, Bulgaria.

ABSTRACT: Gas chromatographic procedure with mass spectrometric detection was applied to quantitatively determine the enzymatic specificity and activity of vipoxin (a neurotoxin from the Vipera ammodytes meridionalis venom) as well as the influence of Ca2+, Mg2+ and Sr2+ on these properties.

KEY WORDS: vipoxin, phospholipase A2, enzyme specificity, Ca2+, Mg2+, Sr2+ influence.

CORRESPONDENCE TO: M. MITEWA, University of Sofia, Faculty of Chemistry, Department of Analytical Chemistry, 1, J. Bourchier Avenue, 1126 Sofia, Bulgaria, Fax: + 3592 96 25 438. Email: mmitewa@chem.uni-sofia.bg