EPIDEMIOLOGIC PROFILE OF BLOOD DONORS FROM ACRE BLOOD CENTER WITH POSITIVE IRREGULAR ANTIBODIES SCREENING

GOLIM M. A.(1), LIMA A. M. P.(2), LIMA C. S.(2), OLIVEIRA E. L. S.(2), SALES L. V. Q.(2), SOARES M. S.(2), TOGNERI M. J. O.(2), ROSSI-FERREIRA R.(1), DEFFUNE E.(1)

(1)Botucatu Blood Center, UNESP, Botucatu, SP, Brazil; (2)Acre Blood Center, Acre, Brazil;

According to the actual hemotherapy law in Brazil, blood donors should be analyzed to define blood type and antibody presence. The detection and identification of irregular antibodies contribute to transfusion safety, avoiding undesirable hemolytic reactions. For describing the epidemiologic profile of blood donors from Acre Blood Center (ABC), Irregular Antibody Screening (IAS) was done by microplate techniques utilizing saline medium, low ionic force buffer and enzymatic medium utilizing papain enzyme. Of 22.976 blood donations received by ABC, from 2002 to 2004, 24 were detected on Irregular Antibodies Research (IAR). Within this 24, IAS positive donors, 19 (79.2%) were included on this study in which 11 (57.9%) were male donors on the age range of 26 to 35 years. Blood transfusion was related by 5 (26.3%) of them, 3 (15.8%) had tattoos 2 (10.5%) related illicit drugs use. All the eight women included on this study related previous pregnancy. Related to irregular antibody screening, the analysis of concordance of results obtained by ABC and Botucatu Blood Center (BBC) revealed 63.2% concordance of results, however, when this comparison was done to the identification of the referred antibodies, 3 (15.7%) were totally concordant, 5 (26.3%) were partially, 2 (10.5%) were non-concordant and in 9 (47.4%) the comparison was not available because one of both hemotherapy centers didn’t identify the specific antibody (ABC or BBC). The most frequent irregular antibodies detected on ABC’s samples by BBC were, anti-D, anti-C, probably anti-c, anti-E, probably anti-Lewis and antibodies for low-frequency antigens, however, in seven serum samples it was impossible to identify. The frequency of IAS positive donors on the studied period was 0.1%, demonstrating lower differences and some divergences among the results found on other Brazilian states. The epidemiologic factors evidenced as major relevance on this research were related to pregnancy history and blood transfusion.

KEY WORDS: blood donors, irregular antibody screening (IAS), haemotherapy

CORRESPONDENCE TO:

Marjorie de Assis Golim, Hemocentro, Distrito Rubião Júnior, s/nº - campus da UNESP, Botucatu, SP, CEP: 18.618-000. Email:marjorie_unesp@yahoo.com.br