SHORT-TERM ACUTE EXERCISE IN LOW AND MODERATE INTENSITIES CHANGES MONOCYTE HISTOPHYSIOLOGY IN RATS.

GUERESCHI M. G.(1), BRANBILLA S. R.(1), DONATTO F. F.(1), PRESTES J.(1), DIAS R.(1), FROLLINI A. B.(1), FERREIRA C. K. O.(1), CAVAGLIERI C. R.(1), PALANCH A. C.(1)

(1)Faculdade de Ciências da Saúde, Universidade Metodista de Piracicaba, SP, Brazil.

Nowadays it is usually described the physical exercise like an improved of health. However, strenuous exercise has been associated to transitory immune suppression, rising infection susceptibility due to stress it causes in organism. Recent studies have described positive effects in innate immune system function associate to exercise. Therefore, the objectives of this study were investigating the effects of short-term low and moderate acute exercise on rat monocyte histophysiology. The experimental groups were divided: sedentary control, Low acute exercise (L) during 5, 10 and 15 minutes and Moderate acute exercise (M) with the same 5, 10 and 15 minutes. The exercise pattern was swimming. The counting of the total circulating monocytes was calculated using the leukocytes total number and differential counting of a blood smear. The monocytes cellular and nuclear area was obtained applying an image analyzer system Image Pro Plus Version 4.0 for Windows. For statistical analysis it was applied ANOVA test followed by Tukey HSD test (p£ 0,05). It was observed an increase in circulating monocyte number in all of the L exercised groups and 5M group, when they were compared with control. Cellular area of blood monocytes presented increase in the groups 5L, 10L, 5M e 10M and the monocyte nuclear area increased in 10L, 5M e 10M. The alterations observed in our study could be due to modulation in synthesis and expression cell protein. Probably, increase in adhesion protein expression. Thereafter, in another way the redistribution of monocytes subsets from the marginal pool could be occurred. Thus, physical exercise in different intensities and volumes can modulate the number and morphology of blood monocytes.

KEY WORDS: morphometry, swimming, innate immune system

FINANCIAL SUPPORT: FAPESP; PIBIC/CNPq; FAP/Unimep.

CORRESPONDENCE TO:

Marcia Grando Guereschi, Faculdade de Ciências da Saúde, Universidade Metodista de Piracicaba, SP, Brasil. E-mail: mgueres@yahoo.com.br