THE ERYTHROID PHENOTYPE OF BLOOD TYPE “O” DONORS IN COORDINATING BLOOD BANK – ACRE STATE – BRAZIL

ROSSI-FERREIRA R.(1); GOLIM M. A.(1); ZAIRE M. C. B.(2); BARROS A.(2); LIMA M. A. M.(2); DEFFUNE E(1)

(1)Botucatu Blood Center, São Paulo, Brazil, (2)Acre Blood Center, Acre - Brazil

The ABO system was discovered in 1990 and remains until today the most important system in transfusional practice. The wrong ABO transfusion can result in death of the patient after a hemolytic reaction followed by immunological and biochemical changes. ABO antibodies are found in the serum of individuals, and are directed against the A and B antigens absent in the red cells. The period between January 26th and February 7th, 111 “O” type blood donors were selected from Acre Coordinator Blood Bank, from both sex and age range of 18 to 61 years to constitute a data bank of phenotypes and make a screening panel. Blood samples were collected without anticoagulant and 0.8% red blood cells suspension was prepared. From this suspension, 50µL were incubated with 25µL of known commercial anti-serum, on microplate, during 15 minutes at 37ºC and centrifuged at 910rpm-10minutes. The erythrocyte phenotype was done for the following systems: RHD (CcEe), Kell, Duffy and for the “N” antigen. From the amount-studied donors, 89.2% were RHD positive that proves the predominance of this antigen on the major of population. Related to RH system, the fenotype Cde/cDe appears on 53.2% of the population. To Kell system, 90.91% are k (Cellano) and 9.09% are Kell positive. The “N” antigen was found in 73.8% of the studied samples. The frequency of Duffy systems reveals the predominance of phenotype Fy (a+b-) diverging from the American Association of Blood Banks – AABB, Technical Manual, probably because of the race diversity in this region. The Kell system and “N” antigen are concordant to AABB.

KEY WORDS: blood donors, phenotype, transfusion

CORRESPONDENCE TO:

Rosana Rossi Ferreira, Hemocentro - Distrito Rubião Júnior, s/nº - campus da UNESP - Botucatu - SP CEP: 18.618-000. Email: rossi@fc.unesp.br