ISOLATION AND CHARACTERIZATION OF NEW PLA2 ISOFORM FROM THE Crotalus durissus collilineatus VENOM.

TOYAMA D.O.(1), ANTUNES, E.(2), CAMARGO E.A. (2), TOYAMA M.H.(3)

(1) Universidade Mackenzie, São Paulo, (2) Departamento de Farmacologia, FMC, UNICAMP, (3) UNESP, CELP, São Vicente

In this article we isolated a new PLA2 isoforms from the Crotalus durissus collilineatus venom collected from the specimens found in the São Paulo and Minas Gerais States.   The venom showed the presence of L-amino acid oxidase that are absent in the venoms from the Goias region.  This PLA2 shows few amino acid replacements in comparison with other PLA2 isolated from this venom.  This protein induce a strong platelet aggregation using dosis of 3-5ug and induced a dose dependent edema using dosis of 3, 6 and 9ug by paw.  Both effects were strongly inhibited by p-bromophenacyl bromide (p-BPB) that induced a not expected strong secondary modification of this protein in solution by circular dicroism.  According these results we observed that p-BPB decreasing of alpha helix and increase of random coils in solution.

KEY WORDS: PLA2, Crotalus durissus collilineatus and circular dicroism.

FINANCIAL SUPPORT: FAPESP, CNPq.