ANTIMICROBIAL ACTIVITY OF SEMI-ÁRIDO SPIDER HAEMOLYMPH Acanthoscurria parahybana (ARANEAE: THERAPHOSIDAE).

FREITAS C. I. A., PAIVA E. S., DIAS E.V., CARMO F.N. A., SERAFIM M.K.B., OLIVEIRA A.F., ROSAS N.S.C., CARMO N.A.

Laboratório de Bioagentes Patogênicos e Imunologia, Departamento de Ciências Animais, Universidade Federal Rural do Semi-Árido, Mossoró, Brasil

The reduction of success in using of antimicrobials against infection diseases causing microbes ultimately leads to an increased risk of acquiring infections in a hospital or other setting. Microbial drug resistance today is posing increasingly serious concerns. One promising answer may be new antimicrobial substances obtained of animal sources that provide the body's of defense mechanisms against pathogenic microorganisms. In this work we used haemolymph of the Brazilian semi-árido spider Acanthoscurria parahybana was obtained by aseptic puncture using anticoagulant rinsed material at 4°C of the dorsal tegument and immediately centrifuged (1). The supernatant were frozen until use. The susceptibility tests by disk diffusion were performed with disk diffusion method in Müeller Hinton agar against Klebsiella pneumoniae ATCC 10031, Enterobacter aerogenes ATCC 1304, Bacillus subtilis ATCC 6633, Escherichia coli ATCC 25922, Salmonella choleraesuis choleraesuis var  typhi ATCC 6534, Pseudomonas aeruginosa HUWC and Staphylococcus aureus ATCC 6538 P. Influences of time, temperature and solubility in antimicrobial activity and toxicity using Artemia salina against different concentrations of haemolymph for 24 hours of evaluation also testing. In our conditions the samples of haemolimph showed a broad spectrum of antimicrobial activity, no significative toxicity and influence of stocking time and heating.

KEY WORDS: antimicrobial activity, spider haemolymph, Acanthoscurria parahybana

CORRESPONDENCE TO:

Carlos Iberê Alves Freitas, Laboratório de Bioagentes Patogênicos e Imunologia, Departamento de Ciências Animais, Universidade Federal Rural do Semi-Árido, BR 110 - Km 47, Bairro Pres. Costa e Silva, Mossoró - Rio Grande do Norte, Brasil, CEP 59625-900, email: ibere@ufersa.edu.br

SILVA JUNIOR, P.I. Sistema imune em aracnídeos: estrutura química e atividade biológica de peptídeos antimicrobianos da hemolinfa da aranha Acanthoscurria gomesiana. São Paulo, Universidade de São Paulo, Instituto de Ciências Biomédicas, 2000. 169 p. [Tese – Doutorado].