EFFECTS OF THE ALGINATES ISOLATED FROM BROWN SEAWEED Sargassum vulgare IN PERFUSED RAT KIDNEY AND MESENTERIC BLOOD VESSELS

ALVES C.D. (3), BARBOSA, P.S.F. (1), SOUSA A.P.A. (1), OLIVEIRA I.M.S. (1), ROCHA I.C.A. (1), MENEZES D.B. (2), COSTA-LOTUFO L.V. (1), ALVES R.S. (1), MARTINS A.M.C. (3), MONTEIRO H.S.A. (1)

(1) Department of Physiology and Pharmacology, (2) Department of Pathology and Legal Medicine/ Federal University of Ceará, (3) Department of Clinical and Toxicologist Analysis/ UFC

Alginates extracted from seaweed at industrial level are widely used in the food industry, biotechnology area and for medical purpose as microencapsulation of hormone-production, treatment of diabetes mellitus and parathyroid disease. The aim of present work was determining the effects of alginates with high viscous (α-L-mannuronate; +V) and low viscous (β-D gluronate; -V) isolated from seaweed in perfused kidney from Wistar rats, as it was described by Fonteles et al., 1983 (1). The effects of 10µg/mL concentration (n=6, each group) were studied on perfusion pressure (PP), renal vascular resistance (RVR), urinary flow (UF), glomerular filtration rate (GFR), sodium (%TNa+), potassium (%TK+) and chloride (%TCl-) tubular transport. All experiments were preceded by a 30 minutes internal control period (C). The data was analyzed by ANOVA and Student t-test (*p<0.05). The -V increased the PP (C = 109.7 ± 0.75 vs -V = 112.15 ± 0.17 mmHg*), RVR (C = 5.76 ± 0.002 vs -V = 0.13 ± 0.004 mmHg.min-1.g-1.min*), UF (C = 0.10 ± 0.01 vs. -V = 0.13 ± 0.004 mL.g-1.min-1*) and GFR (C = 0.761 ± 0.07 vs. -V= 0.422 ± 0.12 mL.g-1.min-1*). However the toxin reduced the %TCl- (C = 79.76 ± 0.56 vs. -V = 59.24 ± 3.15 %*). The +V only changed the PP, RVR and %TCl-. The results of mesenteric blood vessel showed effects only for -V. In conclusion, alginate -V and alginate +V act for different mechanisms. The low viscous alginate probably acts on the endothelial cells and high viscous alginate possibly release of TNFα from mesangial cells in isolated kidney rat.

KEY WORDS: alginates, seaweed, isolated kidney, mesenteric blood vessel.

REFERENCE: (1) FONTELES, M. C. et al. (1983). Am J Physiology 44: 191-197.

FINANCIAL SUPPORT: CNPq.

CORRESPONDENCE TO: MONTEIRO, Helena S. A. Department of Physiology and Pharmacology/ Federal University of Ceará. Phone: +55 85 33668247. Fax: +55 85 33668333. Email: renatasalves@gmail.com