Received: April 24, 2007.

Accepted: June 20, 2007.

Abstract published online: June 6, 2007.

Full paper published online: August 31, 2007.

CONSTRUCTION OF A RECOMBINANT PLASMID pSH-G CONTAINING THE RABIES-VIRUS GLYCOPROTEIN G GENE

CARNIELI JR P. (1), VENTURA A. M. (2), DURIGON E. L. (2)

(1) Pasteur Institute, São Paulo, São Paulo State, Brazil; (2) Department of Microbiology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, São Paulo State, Brazil.

ABSTRACT: A plasmid named pSH-G was constructed with the rabies-virus G-gene insert. This plasmid was transfected into eukaryotic BHK-21 cells and its stability tested. The presence of the pSH-G plasmid was confirmed by means of polymerase chain reaction (PCR) after each of ten cell passages, and the results were positive. The stable BHK-21/pSH-G+ clone obtained can be used in the study of rabies as well as in the production of vaccines.

KEY WORDS: rabies, G gene, plasmids, transfection.

CONFLICTS OF INTEREST: There is no conflict.

CORRESPONDENCE TO:

PEDRO CARNIELI JUNIOR, Instituto Pasteur, Av. Paulista, 393, Cerqueira Cezar, 01311-000, São Paulo, Brasil. Phone: +55 11 3288 0088 ext. 132. Fax: +55 11 3284 4924. Email: pecarniel@yahoo.com.br.