Received: January 12, 2009.

Accepted: March 25, 2009.

Abstract published online: March 31, 2009.

Full paper published online: August 31, 2009.

GENOTYPING OF Clostridium perfringens ASSOCIATED WITH SUDDEN DEATH IN CATTLE

Miyashiro S (1), Baldassi L (1), Nassar AFC (1)

(1) Animal Health Research and Development Center, Biological Institute, São Paulo, São Paulo State, Brazil.

ABSTRACT: Toxigenic types of Clostridium perfringens are significant causative agents of enteric disease in domestic animals, although type E is presumably rare, appearing as an uncommon cause of enterotoxemia of lambs, calves and rabbits. We report herein the typing of 23 C. perfringens strains, by the polymerase chain reaction (PCR) technique, isolated from small intestine samples of bovines that have died suddenly, after manifesting or not enteric or neurological disorders. Two strains (8.7%) were identified as type E, two (8.7%) as type D and the remainder as type A (82.6%). Commercial toxoids available in Brazil have no label claims for efficacy against type E-associated enteritis; however, the present study shows the occurrence of this infection. Furthermore, there are no recent reports on Clostridium perfringens typing in the country.

KEY WORDS: Clostridium perfringens, iota toxin, sudden death, PCR, cattle.

CONFLICTS OF INTEREST: There is no conflict.

CORRESPONDENCE TO:

SIMONE MIYASHIRO, Instituto Biológico, Av. Conselheiro Rodrigues Alves, 1252, Vila Mariana, São Paulo, SP, 04014-002, Brasil. Phone: +55 11 5087 1721. Fax: +55 11 5087 1721. Email: miyashiro@biologico.sp.gov.br.