H-Antiviral activities of extracts and phenolic components of two Spondias species against dengue virus

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J. Venom. Anim. Toxins incl. Trop. Dis.

V.17, n.4, p.406-413, 2011.

Original paper - ISSN 1678-9199.

Antiviral activities of extracts and phenolic components of two Spondias species against dengue virus

Silva ARA (1), Morais SM (1), Marques MMM (1), Lima DM (2), Santos SCC (3), Almeida RR (4), Vieira IGP (4), Guedes MIF (1, 3)

(1) Postgraduate Program in Biotechnology, Ceará State University, Fortaleza, Ceará State, Brazil; (2) Center for Health Sciences, University of Fortaleza, Fortaleza, Ceará State, Brazil; (3) Laboratory of Biochemistry, Ceará State University, Fortaleza, Ceará State, Brazil; (4) Technological Development Park (PADETEC), Fortaleza, Ceará State, Brazil.

Abstract: In recent years, the search for natural plant products to fight viral diseases has been increasing. In this work, two Spondias species, namely S. mombin and S. tuberosa, found in Ceará state (Brazil), and their main phenolic components were evaluated against dengue virus. In vitro antiviral tests were performed against type-2 dengue virus by the MTT method and standard cytopathic effect reduction assay in C6/36 cells. Cytotoxicity was also evaluated by MTT. The presence of phenolic compounds quercetin, rutin, and ellagic acid in plant extracts was characterized by HPLC analysis. Both Spondias species extracts and components were nontoxic to the cells whereas rutin and quercetin displayed relevant antiviral activity with IC50 of 362.68 µg/mL and 500 µg/mL, respectively.

Key words: natural products, phenols, dengue, antiviral agents.

ACKNOWLEDGEMENTS

The authors thank Brazilian government agencies Coordination for the Improvement of Higher Education Personnel (CAPES) and Ceará State Research Foundation (FUNCAP) for their financial support.

SUBMISSION STATUS

Received: February 18, 2011.

Accepted: August 10, 2011.

Abstract published online: August 16, 2011.

Full paper published online: November 30, 2011.

CONFLICTS OF INTEREST

There are no conflicts of interest.

FINANCIAL SOURCE

CAPES and FUNCAP provided the financial grants.

CORRESPONDENCE TO

SELENE MAIA DE MORAIS, Laboratório de Química de Produtos Naturais, Universidade Estadual do Ceará, Fortaleza, CE, 60740-000, Brasil. Phone: +55 85 3101 9789. Fax: +55 85 3101 9933. Email: selene.maia@pq.cnpq.br.