Technetium-99m-labeled deoxynivalenol from Fusarium mycotoxin alters organ toxicity in BALB/c mice by oral and intravenous route

Chattopadhyay P (1), Pandey A (1), Goyary D (1), Chaurasia A (1), Singh L (2), Veer V (1)

(1) Division of Pharmaceutical Technology, Defence Research Laboratory, Assam, India; (2) Department of Life Sciences, Defense Research Development and Organization, New Delhi, India.

Abstract: The toxicity of deoxynivalenol, both intravenously and orally, was investigated in male and female BALB/c mice. Technetium-99m (99m Tc)-labeled deoxynivalenol was administered to mice by tail vein injection and orally dosed. Distribution of labeled deoxynivalenol at 26 hours was monitored by gammascintigraphy. In the evaluated organs, the accumulation of radioactive deoxynivalenol was correlated with the amount of radioactivity. In addition, the toxicity of deoxynivalenol was measured by biochemical assays followed by histopathological findings. Kidney and hepatic marker enzymes were significantly increased in intravenously administered deoxynivalenol as compared to orally treated mice. Intravenously treated mice showed severe damage in liver and kidney when compared to those orally exposed. Biodistribution of 99m Tclabeled deoxynivalenol differed between oral and intravenous treatment. In intravenously exposed mice, deoxynivalenol was distributed primarily in the liver and kidney whereas in oral exposure, it was found in the stomach and intestines after 26 hours. Deoxynivalenol toxicity, associated with its biodistribution and organ toxicity, was greatest where it had accumulated. The results show that the toxicity of deoxynivalenol is associated with organ accumulation.

Key words: technetium-99m (99m Tc), deoxynivalenol, biodistribution, toxicity, histopathology.

Acknowledgments

The authors are grateful to the Defence Research and Development Organization (DRDO), Ministry of Defence, Government of India for financial support.

COPYRIGHT

© CEVAP 2012

SUBMISSION STATUS

Received: September 2, 2012.

Accepted: March 29, 2012.

Abstract published online: April 5, 2012.

Full paper published online: August 31, 2012.

CONFLICTS OF INTEREST

The authors declare no conflicts of interest.

FINANCIAL SOURCE

Defence Research & Development Organization (DRDO), Ministry of Defence, Government of India provided the financial grants.

ETHICS COMMITTEE APPROVAL

The present study was approved by the Institutional Animal Ethics Committee (IAEC) of the Defence Research Laboratory, Tezpur, Assam, India. Moreover, all experimental protocols using animals were performed according to the ”r;Principles of Laboratory Animal Care” (NIH publication 85-23, revised 1985).

CORRESPONDENCE TO

Pronobesh Chattopadhyay, Division of Pharmaceutical Technology, Defence Research Laboratory, Assam, India. Email: chattopadhyay. drdo@gmail.com.