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J. Venom. Anim. Toxins incl. Trop. Dis.

V.20, 2014.

Original paper - ISSN 1678-9199.

 

Gene polymorphism of interleukin 1 and 8 in chronic gastritis patients infected with Helicobacter pylori

 
Agostinho Caleman Neto1, Lucas T Rasmussen2, Roger W de Labio1, Valdeir F de Queiroz3, Marília de AC Smith4, Gustavo A Viani5, Spencer LM Payão1 2 6*

 

1Department of Genetics, FAMEMA Blood Center, Marília Medical School (FAMEMA), Marília, São Paulo State, Brazil

2Sacred Heart University (USC), Bauru, São Paulo State, Brazil

3Department of Digestive System Surgery, Marília Medical School (FAMEMA), Marília, São Paulo State, Brazil

4Department of Morphology, Federal University of São Paulo (UNIFESP), São Paulo, São Paulo State, Brazil

5Department of Radiotherapy and Oncology, Marília Medical School (FAMEMA), Marília, São Paulo State, Brazil

6Laboratório de Genética, Hemocentro, FAMEMA, Rua Lourival Freire, 240, Bairro Fragata, Marília, São Paulo CEP 17519-050, Brazil

 

ABSTRACT

Background

Epidemiological investigations have indicated that Helicobacter pylori induces inflammation in the gastric mucosa regulated by several interleukins. The genes IL1B and IL8 are suggested as key factors in determining the risk of gastritis. The aim of this paper was to evaluate the association of gene polymorphism of interleukin-1 and interleukin-8 with chronic gastrits in H. pylori infected patients. A total of 60 patients underwent endoscopic procedure. Biopsy samples were collected for urease test, histopathological and molecular exams. The DNA of theses samples was extracted for detection of H. pylori and analysis of the genes mentioned above. Patients with gastritis had a higher frequency of H. pylori-positive samples.

 

Results

H. pylori was detected in 30/60 patients (50%) by PCR. As for polymorphism of interleukin 8 (-251) gene we observed a statistical difference when analyzed TA (p = 0.039) and TT (p = 0.047) genotypes. In the IL1B31 there was a statistical difference in TT (p = 0.01) genotype and in theIL1B-511 there wasn’t any statistical difference.

 

Conclusion

Our results suggest a strong correlation between the presence of chronic gastritis and infection by H. pylori and that IL1B-31TT and IL8-251TT genotypes appear to act as protective factors against H. pylori infection while IL8-251TA genotype may comprise a risk factor for infection with this bacterium.

 

Key words: Helicobacter pylori; Polymorphism; Interleukin

 

Ethics committee approval

Patients or legal guardians were informed about the objectives and the research protocol of the present study. They all signed an informed consent form approved by the Research Ethics Committee of Marília Medical School (protocol n. 1119/11).

 

Received: January 7, 2014; Revised: April 2, 2014; Accepted: April 23, 2014

 

Corresponding author: Spencer LM Payão slmpayao@famema.br

 

Competing interests

The authors declare that they have no competing interests.

 

Authors’ contributions

All authors read and approved the final manuscript. ACN carried out the sample collection, DNA extraction and molecular analysis. RWL, LTR, GV, VFQ, MACS, SLMP and ACN participated in the study design, performed the statistical analysis and provided technical support and scientific discussions. SLMP and ACN designed of the study, coordinated the research and helped to draft the manuscript.