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J. Venom. Anim. Toxins incl. Trop. Dis.
V.20, 2014.
Original paper - ISSN 1678-9199.
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J. Venom. Anim. Toxins incl. Trop. Dis. V.20, 2014. Original paper - ISSN 1678-9199. |
Comparative analysis of the venom proteome of four important Malaysian snake species
1Faculty of IndustrialSciences and Technology, University Malaysia Pahang, Lebuhraya Tun Razak, Kuantan, Pahang Darul Makmur 26300, Malaysia
2Institute of Biological Sciences, University of Malaya, Kuala Lumpur 50603, Malaysia.
ABSTRACT
Background
Naja kaouthia, Ophiophagus hannah, Bungarus fasciatus and Calloselasma rhodostoma are four venomous snakes indigenous to Malaysia. In the present study, their proteomic profile by two-dimensional gel electrophoresis (2-DE) have been separated and compared.
Results
The 2-DE of venoms of the four species snake demonstrated complexity and obvious interspecies differences in proteome profiles. A total of 63 proteins were identified in the four species: C. rhodostoma - 26, N. kaouthia - 16, O. hannah - 15 and B. fasciatus - 6.
Conclusions
Despite the identifications of major proteins in the four snake species, a large number of protein spots from the 2-DE were unidentified even though the spots displayed high-quality MALDI-TOF-MS spectra. Those identified included phospholipase A2 proteins in all four venoms, long neurotoxins in both cobra species and the C. rhodostoma venom found with the most varied types of peptidases, i.e. metalloproteinase kistomin, halystase and L-amino acid oxidase.
Key words: Mass spectrometry; Snake venoms; Proteomics; Two-dimensional gel electrophoresis
Received: October 23, 2013; Accepted: February 17, 2014; Revised: March 4, 2014