Interleukin 8 (-251 T>A) polymorphism in children and teenagers infected with Helicobacter pylori

 

Marina Saes¹, Roger Willian de Labio¹, Lucas Trevizani Rasmussen², Spencer Luiz Marques Payão^1 2

 

1 Faculdade de Medicina de Marília (FAMEMA), rua Lourival Freire, 240, Jardim Fragata, CEP 17519-050 Marília, SP, Brazil

 

ABSTRACT

Background

Helicobacter pylori (H. pylori) is a gram-negative bacterium that colonizes the human stomach and causes a variety of gastric diseases. This study evaluated the correlations between the -251 (T>A) (rs4073) polymorphism of interleukin-8 (IL-8), the etiology of gastric disease, and H. pylori infection in pediatric and adolescent patients.

Methods

DNA samples were obtained from 285 gastric biopsies from pediatric patients. H. pylori was detected by PCR, whereas PCR-RFLP was used to characterize the -251 (T>A) polymorphism of IL-8.

Results

The histological analysis revealed the presence of gastritis in 158 patients (55.44%). H. pylori was found in 71 samples (24.9%). The -251 (T>A) polymorphism revealed that 58 (29.47%) samples were TT, 143 (50.18%) samples were TA, and 84 (20.35%) samples were AA.

Conclusions

Our findings suggest that IL8-251 A allele may be an important risk factor for the development of gastric disease when associated with H. pylori infection.

 

Key words: Helicobacter pylori; Interleukin 8; Polymorphism; -251 (T>A); Gastric disease

 

Funding

Not applicable.

 

Received: October 18, 2016.

Revised: April 1, 2017.

Accepted: April 8, 2017.

 

Correspondence: slmpayao@famema.br

 

Authors’ contributions

MS, LTR, and SLMP wrote the first draft of the manuscript and revised it. MS, LTR, and RWL performed all of the experiments. MS and LTR carried out the analyses. SLMP helped in designing the experiments and corrected the final version of article. All authors read and approved the final manuscript.

 

Competing interests

The authors declare that they have no competing interests.

 

Consent for publication

All patients and legal guardians were informed of the research protocol and signed an informed consent form.

 

Ethics approval and consent to participate

The present study was approved by the Research Ethics Committee of Marilia Medical School (protocol no. 1119/11).

 

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