Interleukin 8 (-251 T>A) polymorphism in children and teenagers infected with Helicobacter pylori
Marina Saes1 , Roger Willian de Labio1 , Lucas Trevizani Rasmussen2 and Spencer Luiz Marques Payão1,2* [ + show more ]
Received: 18 October 2016 | Accepted: 1 April 2017 | Published: 08 April 2017
https://doi.org/10.1186/s40409-017-0113-z
Abstract
Background: Helicobacter pylori (H. pylori) is a gram-negative bacterium that colonizes the human stomach and causes a variety of gastric diseases. This study evaluated the correlations between the -251 (T>A) (rs4073) polymorphism of interleukin-8 (IL-8), the etiology of gastric disease, and H. pylori infection in pediatric and adolescent patients. Methods: DNA samples were obtained from 285 gastric biopsies from pediatric patients. H. pylori was detected by PCR, whereas PCR-RFLP was used to characterize the -251 (T>A) polymorphism of IL-8. Results: The histological analysis revealed the presence of gastritis in 158 patients (55.44%). H. pylori was found in 71 samples (24.9%). The -251 (T>A) polymorphism revealed that 58 (29.47%) samples were TT, 143 (50.18%) samples were TA, and 84 (20.35%) samples were AA. Conclusions: Our findings suggest that IL8-251 A allele may be an important risk factor for the development of gastric disease when associated with H. pylori infection.
Keywords: Helicobacter pylori, Interleukin 8, Polymorphism, -251 (T>A), Gastric disease